In a convenience store, new items like Butterfly Wooden Plaque lunch boxes and dairy products are delivered several times in one day.In addition, old unsold items must be disposed because of their expiration dates.The order is made once in a day, and the retailer has to decide the number of items ordered based on the current remaining items in the
Summary: Off-target binding and cleavage by Cas9 pose major challenges in its application.How the conformational dynamics of Cas9 govern its nuclease activity under on- and off-target conditions remains largely unknown.Here, using intra-molecular single-molecule fluorescence resonance energy transfer measurements, we revealed that Cas9 in Balloons
Trindade is an oceanic island located ca.1200 km east of the Brazilian coast with only 65% of its original vegetation cover remaining.Devastation was mainly caused by the presence of goats for Countertop Oven a long time.Since goats were eliminated in 2005 the island vegetation is going through a recovering process.However during a visit to the isl
Laccase is a multi-copper enzyme widely expressed in fungi, higher plants, and bacteria which facilitates the direct reduction of molecular oxygen to water (without hydrogen peroxide production) accompanied by the oxidation of an electron donor.Laccase has attracted attention in biotechnological applications due to its non-specificity and use of mo